Abstract
A fucose-containing glycoprotein fraction which stimulates spleen cell proliferation and cytokine expression has been identified from the water-soluble extract of Ganoderma lucidum. Proteomic analysis of mouse spleen cells treated with this glycoprotein fraction showed approximately 50% change of the proteome. Further studies on the activities of this glycoprotein fraction through selective proteolysis and glycosidic cleavage indicate that a fucose containing polysaccharide fraction is responsible for stimulating the expression of cytokines, especially IL-1, IL-2 and INF-gamma.
MeSH terms
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Adjuvants, Immunologic / analysis
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Adjuvants, Immunologic / pharmacology*
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Animals
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Antineoplastic Agents / analysis
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Antineoplastic Agents / pharmacology*
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Carbohydrate Sequence
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DNA Fingerprinting
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Drugs, Chinese Herbal / chemistry
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Epitopes / immunology
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Fucose
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Glycoproteins / analysis
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Glycoproteins / pharmacology
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Male
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Polysaccharides / analysis
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Polysaccharides / pharmacology*
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Reishi / chemistry*
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Spleen / cytology
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Spleen / drug effects
Substances
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Adjuvants, Immunologic
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Antineoplastic Agents
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Drugs, Chinese Herbal
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Epitopes
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Glycoproteins
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Polysaccharides
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Fucose